Maciek R. Antoniewicz

• PhD, Massachusetts Institute of Technology (2006), Chemical Engineering
• MS, Delft University of Technology (2000), Chemical Engineering
• BS, Delft University of Technology (1998), Chemical Engineering

• Metabolic engineering
• Biotechnology
• Cancer, metabolism
• Microbial communities
• Quantifying in vivo fluxes
• Engineering metabolic pathways
• Adaptive evolution
• Systems biology

The Antoniewicz lab is developing next-generation tools and techniques for analyzing, engineering and manipulating microbial and mammalian cells applied to specific problems in biotechnology (biofuels, pharmaceuticals) and medicine (cancer, obesity, diabetes). In our research we make use of modern techniques for cell culture, 13C metabolic flux analysis, mass spectrometry, molecular biology, bioinformatics and computational biology. Current interests include: elucidating syntrophic interactions in microbial communities, analysis of compartment-specific fluxes in mammalian cells, dynamic flux analysis, cancer metabolism, and engineering microbes for enhanced utilization of renewable substrates and production of value-added chemicals.

• Elected Fellow, American Institute for Medical and Biological Engineering (AIMBE), 2018.
• National Academy of Engineering (NAE) US-EU, Frontiers of Engineering Participant, 2017.
• Biotechnology and Bioengineering (B&B) Daniel I.C. Wang Award, 2015.
• Gerard J. Mangone Best Young Scholar Award, University of Delaware, 2012.
• Outstanding Junior Faculty Member, College of Engineering, University of Delaware, 2012.
• National Science Foundation (NSF) CAREER Award, 2011.
• James E. Bailey Young Investigator Award in Metabolic Engineering, 2008.
• DuPont Young Professor Award, 2008

Selected Journal Articles

• For a more complete list see Google Scholar
• Long CP, Antoniewicz MR. “High-resolution 13C metabolic flux analysis”. Nature Protocols, 2019.
• Long CP, Antoniewicz MR. “Metabolic flux responses to deletion of 20 core enzymes reveal flexibility and limits of E. coli metabolism”. Metabolic Engineering, 55: 249–257, 2019.
• Antoniewicz MR. “Synthetic methylotrophy: Strategies to assimilate methanol for growth and chemicals production”. Current Opinion Biotechnology, 59: 165–174, 2019.
• Long CP, Antoniewicz MR. “How adaptive evolution reshapes metabolism to improve fitness: recent advances and future outlook”. Current Opinion Chemical Engineering, 22: 209-215, 2018.
• Antoniewicz MR. “A guide to 13C metabolic flux analysis for the cancer biologist”. Experimental & Molecular Medicine, 50(4): 19, 2018.
• DeWaal D, Nogueira V, Terry AR, Patra CP, Jeon SM, Guzman G, Au J, Long CP, Antoniewicz MR, Hay N. “Hexokinase-2 depletion inhibits glycolysis and induces oxidative phosphorylation in hepatocellular carcinoma and sensitizes to metformin”. Nature Communications, 9:446, 2018.
• Long CP, Gonzalez JE, Feist AM, Palsson BO, Antoniewicz MR. “Dissecting the genetic and metabolic mechanisms of adaptation to the knockout of a major metabolic enzyme in Escherichia coli”. PNAS, 115(1): 222-227, 2018.
• Long CP, Au J, Sandoval NR, Gebreselassie NA, Antoniewicz MR. “Enzyme I facilitates reverse flux from pyruvate to phosphoenolpyruvate in Escherichia coli”. Nature Communications, 8:14316, 2017.
• Jones SW, Fast AG, Carlson ED, Wiedel CA, Au J, Antoniewicz MR, Papoutsakis ET, Tracy BP. “CO2 fixation by anaerobic non-photosynthetic mixotrophy for improved carbon conversion”. Nature Communications, 7:12800, 2016.
• Ahn WS, Antoniewicz MR. “Parallel labeling experiments with [1,2-13C]glucose and [U-13C]glutamine provide new insights into CHO cell metabolism”. Metabolic Engineering, 15: 34-47, 2013.